Quantitative PCR confirms culture as the gold standard for detection of lower airway infection by nontypeable Haemophilus influenzae in Australian Indigenous children with bronchiectasis.

Hare, Kim; Marsh, Robyn; Binks, Michael; Grimwood, Keith; Pizzutto, Susan; Leach, Amanda; Chang, Anne; Smith-Vaughan, Heidi

doi:10.1016/j.mimet.2012.12.013

Title

Quantitative PCR confirms culture as the gold standard for detection of lower airway infection by nontypeable Haemophilus influenzae in Australian Indigenous children with bronchiectasis.

Link to article in PubMed

https://pubmed.ncbi.nlm.nih.gov/23266579/?otool=iaurydwlib

Author(s)

Hare, Kim

Marsh, Robyn

Binks, Michael

Grimwood, Keith

Pizzutto, Susan

Leach, Amanda

Chang, Anne

Smith-Vaughan, Heidi

Abstract

Correlation was observed between quantitative PCR and semi-quantitative culture for definition of Haemophilus influenzae infection in bronchoalveolar lavage specimens from 81 children with bronchiectasis. However, qPCR data correlated less well with airway neutrophilia, and supports continued use of culture as the gold standard for defining H. influenzae lower airway infection.

Publication information

Microbiol Methods . 2013 Mar;92(3):270-2. doi: 10.1016/j.mimet.2012.12.013. Epub 2012 Dec 22.

Date Issued

2013-03-01