| Title |
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Enterotoxigenic Escherichia coli in Central Australia: diagnosis using cloned and synthetic nucleic acid probes. |
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| Publication Date |
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| Author(s) |
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| Affiliation |
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Division of Clinical Microbiology, Institute of Medical and Veterinary Science, Adelaide. |
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| MESH subject |
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Escherichia coli Infections |
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Nucleic Acid Hybridization |
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| Type of document |
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| Entity Type |
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| Abstract |
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Feces from 169 children admitted with diarrhea to Alice Springs Hospital, were screened for enterotoxigenic E. coli (ETEC) using specific DNA probes. E. coli which hybridized with probes for ST-P, ST-H and LT were confirmed by bioassay for toxin production. Fifty children were shown to excrete ETEC. The probes for LT correlated well with bioassay; however, probes for ST-H and ST-P hybridized with more E. coli than were shown to produce toxin by bioassay. When probing for ST was repeated using a synthetic oligonucleotide probe, only those specimens which were bioassay-positive hybridized with the probe. |
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| Link |
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| Citation |
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Pathology . 1988 Apr;20(2):167-72. doi: 10.3109/00313028809066628. |
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| ISSN |
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| Pubmed ID |
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https://pubmed.ncbi.nlm.nih.gov/3062556/?otool=iaurydwlib |
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