Towards a rapid molecular diagnostic for melioidosis: Comparison of DNA extraction methods from clinical specimens.

Richardson, Leisha; Kaestli, Mirjam; Mayo, Mark; Bowers, Jolene; Tuanyok, Apichai; Schupp, Jim; Engelthaler, David; Wagner, David; Keim, Paul; Currie, Bart

doi:10.1016/j.mimet.2011.10.023

Title

Towards a rapid molecular diagnostic for melioidosis: Comparison of DNA extraction methods from clinical specimens.

Link to article in PubMed

https://pubmed.ncbi.nlm.nih.gov/22108495/?otool=iaurydwlib

Author(s)

Richardson, Leisha

Kaestli, Mirjam

Mayo, Mark

Bowers, Jolene

Tuanyok, Apichai

Schupp, Jim

Engelthaler, David

Wagner, David

Keim, Paul

Currie, Bart

Abstract

Optimising DNA extraction from clinical samples for Burkholderia pseudomallei Type III secretion system real-time PCR in suspected melioidosis patients confirmed that urine and sputum are useful diagnostic samples. Direct testing on blood remains problematic; testing DNA extracted from plasma was superior to DNA from whole blood or buffy coat.

Publication information

J Microbiol Methods . 2012 Jan;88(1):179-81. doi: 10.1016/j.mimet.2011.10.023. Epub 2011 Nov 12.

Date Issued

2012-01-01