Please use this identifier to cite or link to this item: https://hdl.handle.net/10137/8560
Title: The Aetiology of pneumonia from analysis of Lung aspirate and Pleural fluid samples: Findings from the PERCH study.
Authors: Ebruke, Bernard E
Knoll, Maria Deloria
Haddix, Meredith
Zaman, Syed M A
Prosperi, Christine
Feikin, Daniel R
Hammitt, Laura L
Levine, Orin S
O'Brien, Katherine L
R Murdoch, David
Brooks, W Abdullah
Scott, J Anthony G
Kotloff, Karen L
Madhi, Shabir A
Thea, Donald M
Baillie, Vicky L
Chisti, Mohammod Jobayer
Dione, Michel
J Driscoll, Amanda
Fancourt, Nicholas
Karron, Ruth A
Le, Tham T
Mohamed, Shebe
Moore, David P
C Morpeth, Susan
Mwaba, John
Mwansa, James
Shahid, Abu Sadat Mohammad Sayeem Bin
Sow, Samba O
Tapia, Milagritos D
Antonio, Martin
Howie, Stephen R C
Citation: © The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America.
Clin Infect Dis. 2020 Jul 25:ciaa1032. doi: 10.1093/cid/ciaa1032.
Abstract: BACKGROUND: An improved understanding of childhood pneumonia aetiology is required to inform prevention and treatment strategies. Lung aspiration is the gold standard specimen for pneumonia diagnostics. We report findings from analyses of lung and pleural aspirates collected in the Pneumonia Etiology Research for Child Health (PERCH) study. METHODS: The PERCH study enrolled children aged 1-59 months hospitalized with World Health Organization defined severe or very severe pneumonia in 7 countries in Africa and Asia. Percutaneous trans-thoracic lung (LA) and pleural fluid (PF) aspiration was performed on a sample of pneumonia cases with radiological consolidation and/or pleural fluid in 4 countries. Venous blood and nasopharyngeal/oropharyngeal swabs were collected from all cases. Multiplex quantitative PCR and routine microbiologic culture were applied to clinical specimens. RESULTS: Of 44 LAs performed within 3 days of admission on 622 eligible cases, 13 (30%) had a pathogen identified by either culture (5/44) or by PCR (11/29). A pathogen was identified in 12/14 (86%) PF specimens tested by either culture (9/14) or PCR (9/11). Bacterial pathogens were identified more frequently than viruses. All but one of the cases with a virus identified were co-infected with bacterial pathogens. Streptococcus pneumoniae (9/44 [20%]) and Staphylococcus aureus (7/14 [50%]) were the predominant pathogen identified in LA and PF, respectively. CONCLUSIONS: Bacterial pathogens predominated in this selected subgroup of PERCH participants drawn from those with radiological consolidation or pleural fluid, with S. pneumoniae and S. aureus the leading pathogens identified.
Click to open Pubmed Article: https://www.ezpdhcs.nt.gov.au/login?url=https://www.ncbi.nlm.nih.gov/pubmed/32710751
Journal title: Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
Publication Date: 2020-07-25
Type: Journal Article
URI: https://hdl.handle.net/10137/8560
DOI: 10.1093/cid/ciaa1032
Appears in Collections:(a) NT Health Research Collection

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