Please use this identifier to cite or link to this item: https://hdl.handle.net/10137/7727
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dc.contributor.authorWebb JRen
dc.contributor.authorRachlin Aen
dc.contributor.authorRigas Ven
dc.contributor.authorSarovich DSen
dc.contributor.authorPrice EPen
dc.contributor.authorKaestli Men
dc.contributor.authorWard LMen
dc.contributor.authorMayo MJen
dc.contributor.authorCurrie BJen
dc.date2019en
dc.date.accessioned2019-08-06T01:54:54Zen
dc.date.available2019-08-06T01:54:54Zen
dc.date.issued2019-07-26en
dc.identifier.citationPLoS neglected tropical diseases 2019-07-26; 13(7): e0007369en
dc.identifier.urihttps://hdl.handle.net/10137/7727en
dc.description.abstractThe Tier 1 select agent Burkholderia pseudomallei is an environmental bacterium that causes melioidosis, a high mortality disease. Variably present genetic markers used to elucidate strain origin, relatedness and virulence in B. pseudomallei include the Burkholderia intracellular motility factor A (bimA) and filamentous hemagglutinin 3 (fhaB3) gene variants. Three lipopolysaccharide (LPS) O-antigen types in B. pseudomallei have been described, which vary in proportion between Australian and Asian isolates. However, it remains unknown if these LPS types can be used as genetic markers for geospatial analysis within a contiguous melioidosis-endemic region. Using a combination of whole-genome sequencing (WGS), statistical analysis and geographical mapping, we examined if the LPS types can be used as geographical markers in the Northern Territory, Australia. The clinical isolates revealed that LPS A prevalence was highest in the Darwin and surrounds (n = 660; 96% being LPS A and 4% LPS B) and LPS B in the Katherine and Katherine remote and East Arnhem regions (n = 79; 60% being LPS A and 40% LPS B). Bivariate logistics regression of 999 clinical B. pseudomallei isolates revealed that the odds of getting a clinical isolate with LPS B was highest in East Arnhem in comparison to Darwin and surrounds (OR 19.5, 95% CI 9.1-42.0; p<0.001). This geospatial correlation was subsequently confirmed by geographically mapping the LPS type from 340 environmental Top End strains. We also found that in the Top End, the minority bimA genotype bimABm has a similar remote region geographical footprint to that of LPS B. In addition, correlation of LPS type with multi-locus sequence typing (MLST) was strong, and where multiple LPS types were identified within a single sequence type, WGS confirmed homoplasy of the MLST loci. The clinical, sero-diagnostic and vaccine implications of geographically-based B. pseudomallei LPS types, and their relationships to regional and global dispersal of melioidosis, require global collaborations with further analysis of larger clinically and geospatially-linked datasets.en
dc.language.isoengen
dc.titleTracing the environmental footprint of the Burkholderia pseudomallei lipopolysaccharide genotypes in the tropical "Top End" of the Northern Territory, Australia.en
dc.typeJournal Articleen
dc.identifier.journaltitlePLoS neglected tropical diseasesen
dc.identifier.doi10.1371/journal.pntd.0007369en
dc.identifier.pubmedidhttps://www.ezpdhcs.nt.gov.au/login?url=https://www.ncbi.nlm.nih.gov/pubmed//31348781en
dc.identifier.orcidhttp://orcid.org/0000-0002-6332-039Xen
dc.identifier.orcidhttp://orcid.org/0000-0002-8878-8837en
dc.identifier.affiliationGlobal and Tropical Health Division, Menzies School of Health Research, Darwin, Northern Territory, Australia..en
dc.identifier.affiliationGlobal and Tropical Health Division, Menzies School of Health Research, Darwin, Northern Territory, Australia..en
dc.identifier.affiliationGlobal and Tropical Health Division, Menzies School of Health Research, Darwin, Northern Territory, Australia..en
dc.identifier.affiliationGlobal and Tropical Health Division, Menzies School of Health Research, Darwin, Northern Territory, Australia.. Genecology Research Centre, University of the Sunshine Coast, Sippy Downs, Queensland, Australia..en
dc.identifier.affiliationGlobal and Tropical Health Division, Menzies School of Health Research, Darwin, Northern Territory, Australia.. Genecology Research Centre, University of the Sunshine Coast, Sippy Downs, Queensland, Australia..en
dc.identifier.affiliationGlobal and Tropical Health Division, Menzies School of Health Research, Darwin, Northern Territory, Australia.. Research Institute for the Environment and Livelihoods, Charles Darwin University, Darwin, Northern Territory, Australia..en
dc.identifier.affiliationGlobal and Tropical Health Division, Menzies School of Health Research, Darwin, Northern Territory, Australia..en
dc.identifier.affiliationGlobal and Tropical Health Division, Menzies School of Health Research, Darwin, Northern Territory, Australia..en
dc.identifier.affiliationGlobal and Tropical Health Division, Menzies School of Health Research, Darwin, Northern Territory, Australia.. Department of Infectious Diseases and Northern Territory Medical Program, Royal Darwin Hospital, Darwin, Northern Territory, Australia..en
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