Please use this identifier to cite or link to this item: https://hdl.handle.net/10137/5803
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dc.contributor.authorShemesh, Tomeren
dc.contributor.authorKarschimkus, Connieen
dc.contributor.authorRowley, Kevin Gen
dc.date2005en
dc.date.accessioned2018-05-15T23:01:55Zen
dc.date.available2018-05-15T23:01:55Zen
dc.date.issued2005-11-05en
dc.identifier.citationJournal of chromatography. B, Analytical technologies in the biomedical and life sciences 2005-11-05; 826(1-2): 277-8en
dc.identifier.issn1570-0232en
dc.identifier.urihttps://hdl.handle.net/10137/5803en
dc.description.abstractSemicarbazide-sensitive amine oxidase (SSAO; EC 1.4.3.6) is a copper-containing enzyme predominantly expressed by vascular smooth muscle cells. SSAO deaminates primary amines to produce aldehydes and oxygen peroxides, and may thus play a role in vascular damage. SSAO activity can be quantified by assaying benzaldehyde production using fluorescent derivatisation and separation by HPLC. We performed the derivatisation step in polypropylene or borosilicate glass tubes over 45 min at 95 degrees C. High and obstructing background levels of benzaldehyde were found in one batch of polypropylene vials, as opposed to its alternatives. Treatment and handling of product shipment into the country did not account for introduction of contaminant into packaged vials nor did any reagent used in the assay. We conclude that the source of contamination was most likely due to variation in the commercial production process. Use of borosilicate vials for assays based on aldehyde production and derivatisation is recommended.en
dc.language.isoengen
dc.titleContamination in HPLC quantified benzaldehyde is from polypropylene microtubes.en
dc.typeJournal Articleen
dc.identifier.journaltitleJournal of chromatography. B, Analytical technologies in the biomedical and life sciencesen
dc.identifier.doi10.1016/j.jchromb.2005.09.003en
dc.identifier.pubmedidhttps://www.ezpdhcs.nt.gov.au/login?url=https://www.ncbi.nlm.nih.gov/pubmed//16188506en
dc.subject.meshAmine Oxidase (Copper-Containing)en
dc.subject.meshBenzaldehydesen
dc.subject.meshChromatography, High Pressure Liquiden
dc.subject.meshGlassen
dc.subject.meshHumansen
dc.subject.meshEquipment Contaminationen
dc.subject.meshPolypropylenesen
dc.identifier.affiliationMenzies School of Health Research, Institute of Advanced Studies, Charles Darwin University, John Mathews Building, Royal Darwin Hospital, Rocklands Drive Tiwi NT 0810, Australia. tomer.shemesh@menzies.edu.au.en
dc.identifier.pubmedurihttps://www.ezpdhcs.nt.gov.au/login?url=https://www.ncbi.nlm.nih.gov/pubmed//16188506en
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