Please use this identifier to cite or link to this item: https://hdl.handle.net/10137/5579
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Title: Quantification of Plasmodium falciparum histidine-rich protein-2 in cerebrospinal spinal fluid from cerebral malaria patients.
Authors: Mikita, Kei
Thakur, Kiran
Anstey, Nicholas M
Piera, Kim A
Pardo, Carlos A
Weinberg, J Brice
Mukemba, Jackson
Florence, Salvatore
Mwaikambo, Esther D
Granger, Donald L
Sullivan, David J
Citation: The American journal of tropical medicine and hygiene 2014-09; 91(3): 486-92
Abstract: A cerebrospinal fluid (CSF) biomarker for cerebral malaria (CM) has not been validated. We examined the detection, semiquantification, and clinical use of the Plasmodium falciparum histidine-rich protein-2 (PfHRP-2) as a parasite antigen biomarker for CM. The PfHRP-2 was detected in archival CSF samples from CM patients from Tanzania both by a newly developed sensitive and specific immuno-polymerase chain reaction (72 of 73) and by rapid diagnostic tests (62 of 73). The geometric mean PfHRP-2 CSF concentration was 8.76 ng/mL with no differences in those who survived (9.2 ng/mL), those who died (11.1 ng/mL), and those with neurologic sequelae (10.8 ng/mL). All aparasitemic endemic and nonendemic control samples had undetectable CSF PfHRP-2. In a separate group of 11 matched plasma and CSF cerebral malaria patient samples, the ratio of plasma to CSF PfHRP-2 was 175. The CSF PfHRP-2 reflects elevated plasma PfHRP-2 rather than elevated CM-specific CSF ratios, falling short of a validated biomarker.
Click to open PubMed article: https://www.ezpdhcs.nt.gov.au/login?url=https://www.ncbi.nlm.nih.gov/pubmed/24980497
Click to open Pubmed Article: https://www.ezpdhcs.nt.gov.au/login?url=https://www.ncbi.nlm.nih.gov/pubmed/24980497
Journal title: The American journal of tropical medicine and hygiene
Publication Date: 2014-09
Type: Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
URI: https://hdl.handle.net/10137/5579
DOI: 10.4269/ajtmh.14-0210
Appears in Collections:(a) NT Health Research Collection

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