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Title: Whole-genome sequencing to investigate a non-clonal melioidosis cluster on a remote Australian island.
Authors: Sarovich DS
Chapple SNJ
Price EP
Mayo MJ
Holden MTG
Peacock SJ
Currie BJ
Citation: Microbial genomics 2017-08; 3(8): e000117
Abstract: Melioidosis is a tropical disease caused by the bacterium Burkholderia pseudomallei. Outbreaks are uncommon and can generally be attributed to a single point source and strain. We used whole-genome sequencing to analyse B. pseudomallei isolates collected from an historical 2-year long case cluster that occurred in a remote northern Australian indigenous island community, where infections were previously linked to a contaminated communal water supply. We analysed the genome-wide relatedness of the two most common multilocus sequence types (STs) involved in the outbreak, STs 125 and 126. This analysis showed that although these STs were closely related on a whole-genome level, they demonstrated evidence of multiple recombination events that were unlikely to have occurred over the timeframe of the outbreak. Based on epidemiological and genetic data, we also identified two additional patients not previously associated with this outbreak. Our results confirm the previous hypothesis that a single unchlorinated water source harbouring multiple B. pseudomallei strains was linked to the outbreak, and that increased melioidosis risk in this community was associated with Piper methysticum root (kava) consumption.
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Journal title: Microbial genomics
Publication Date: 2017-08
Type: Journal Article
DOI: 10.1099/mgen.0.000117
Appears in Collections:(a) NT Health Research Collection

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