Please use this identifier to cite or link to this item: https://hdl.handle.net/10137/11440
Title: Is Streptococcus pyogenes resistant or susceptible to trimethoprim-sulfamethoxazole?
Authors: Bowen AC
Lilliebridge RA
Tong SYC
Baird RW
Ward P
McDonald MI
Currie BJ
Carapetis JR
Citation: J Clin Microbiol. 2012 Dec;50(12):4067-72. doi: 10.1128/JCM.02195-12. Epub 2012 Oct 10.
Abstract: Streptococcus pyogenes is commonly believed to be resistant to trimethoprim-sulfamethoxazole (SXT), resulting in reservations about using SXT for skin and soft tissue infections (SSTI) where S. pyogenes is involved. S. pyogenes' in vitro susceptibility to SXT depends on the medium's thymidine content. Thymidine allows S. pyogenes to bypass the sulfur-mediated inhibition of folate metabolism and, historically, has resulted in apparently reduced susceptibility of S. pyogenes to sulfur antibacterials. The low thymidine concentration in Mueller-Hinton agar (MHA) is now regulated. We explored S. pyogenes susceptibility to SXT on various media. Using two sets of 100 clinical S. pyogenes isolates, we tested for susceptibility using SXT Etests on MHA containing defibrinated horse blood and 20 mg/liter β-NAD (MHF), MHA with sheep blood (MHS), MHA alone, MHA with horse blood (MHBA), and MHA with lysed horse blood (MHLHBA). European Committee on Antibacterial Susceptibility Testing (EUCAST) breakpoints defined susceptibility (MIC, ≤ 1 mg/liter) and resistance (MIC, >2 mg/liter). In study 1, 99% of S. pyogenes isolates were susceptible to SXT on MHA, MHBA, and MHLHBA, with geometric mean MICs of 0.04, 0.04, and 0.05 mg/liter, respectively. In study 2, all 100 S. pyogenes isolates were susceptible to SXT on MHF, MHS, MHA, and MHLHBA with geometric mean MICs of 0.07, 0.16, 0.07, and 0.09 mg/liter, respectively. This study confirms the in vitro susceptibility of S. pyogenes to SXT, providing support for the use of SXT for SSTIs. A clinical trial using SXT for impetigo is ongoing.
Click to open Pubmed Article: https://www.ezpdhcs.nt.gov.au/login?url=https://www.ncbi.nlm.nih.gov/pubmed/23052313
Journal title: Journal of clinical microbiology
Volume: 50
Pages: 4067-72
Publication Date: 2012-12
Type: Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't
URI: https://hdl.handle.net/10137/11440
DOI: 10.1128/JCM.02195-12
Appears in Collections:(a) NT Health Research Collection

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