Jervis-Bardy J

Rogers GB

Morris PS

Smith-Vaughan HC

Nosworthy E

Leong LEX

Smith RJ

Weyrich LS

De Haan J

Carney AS

Leach AJ

O'Leary S

Marsh RL

2015-09

Indigenous Australian children have a high prevalence of otitis media with effusion (OME) and associated conductive hearing loss. Only three microbiological studies of middle ear fluid (MEF) from Indigenous Australian children with OME have been reported. All of these were reliant on culture or species-specific PCR assays. The aim of this study was to characterise the middle ear fluid (MEF), adenoid and nasopharyngeal (NP) microbiomes of Indigenous Australian children, using culture-independent 16S rRNA gene sequencing. MEF, NP swabs and adenoid specimens were collected from 11 children in the Alice Springs region of Central Australia. Bacterial communities in these specimens were characterised using 16S rRNA gene sequencing. The microbiota in MEF samples were dominated (>50% relative abundance) by operational taxonomic units (OTUs) consistent with Alloiococcus otitidis (6/11), Haemophilus influenzae (3/11) or Streptococcus sp. (specifically, Mitis group streptococci which includes Streptococcus pneumoniae) (1/11). Anatomical site selectivity was indicated by the presence of a single conserved Haemophilus OTU in 7/11 MEF samples. In comparison, there were ten distinct Haemophilus OTUs observed across the NP and adenoid samples. Despite significant differences between the MEF and NP/adenoid microbiomes, Streptococcus sp., H. influenzae and Moraxella catarrhalis OTUs were common to all sample types. Co-occurrence of classical otopathogens in paired MEF and NP/Adenoid samples is consistent with earlier culture-based studies. These data highlight the need to further assess H. influenzae traits important in otitis media and to understand the role of canal flora, especially A. otitidis, in populations with a high prevalence of tympanic membrane perforation.

International journal of pediatric otorhinolaryngology 2015-09; 79(9): 1548-55

https://pubmed.ncbi.nlm.nih.gov/26228497/?otool=iaurydwlib

https://hdl.handle.net/10137/5453

16S ribosomal RNA

Indigenous

Microbiome

Middle ear effusion

Otitis media

Adenoids

Australia

Carnobacteriaceae

Child

Child, Preschool

Ear, Middle

Female

Haemophilus influenzae

Humans

Male

Microbiota

Moraxella (Branhamella) catarrhalis

Nasopharynx

Oceanic Ancestry Group

Otitis Media with Effusion

RNA, Bacterial

RNA, Ribosomal, 16S

Streptococcus

The microbiome of otitis media with effusion in Indigenous Australian children.

Journal Article

Publication