Kaestli, Mirjam

Farook, Saika

Jilani, Md Shariful Alam

Anwar, Shaheda

Siddiqui, Tanvir Ahmed

Mayo, Mark

Podin, Yuwana

Webb, Jessica R

Dance, David A B

Currie, Bart

2024-09-03

Melioidosis is an emerging tropical infectious disease with a rising global burden caused by the environmental bacterium Burkholderia pseudomallei. It is endemic in Southeast and South Asia, including Bangladesh. A rare aminoglycoside-susceptible B. pseudomallei isolate (Y2019) has recently been reported from a melioidosis patient in Dhaka, Bangladesh. To understand the geographical origins of Y2019, we subjected it and 10 other isolates from Bangladesh to whole-genome sequencing. In a phylogenetic tree with a global set of B. pseudomallei genomes, most Bangladeshi genomes clustered tightly within the Asian clade. In contrast, Y2019 was closely related to ST881 isolates from Sarawak, Malaysian Borneo, a gentamicin-sensitive sequence type, suggesting infection in Borneo. Y2019 also contained the same gentamicin sensitivity conferring nonsynonymous mutation in the drug efflux pump encoding the amrB gene. In the absence of a full travel history, whole-genome sequencing and bioinformatics tools have revealed the likely origin of this rare isolate.

Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia.

Department of Microbiology, Ibrahim Medical College, Dhaka, Bangladesh.

Department of Microbiology, Ibrahim Medical College, Dhaka, Bangladesh.

Department of Microbiology and Immunology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh.

Department of Microbiology and Immunology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh.

Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia.

Institute of Health and Community Medicine, Universiti Malaysia Sarawak, Sarawak, Malaysia.

Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia.

Department of Microbiology and Immunology at The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia.

Lao-Oxford-Mahosot Hospital-Wellcome Trust Unit, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic.

Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom.

Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia.

Infectious Diseases Department, Royal Darwin Hospital, Darwin, Northern Territory, Australia.

Am J Trop Med Hyg . 2024 Sep 3;111(5):1056-1059. doi: 10.4269/ajtmh.24-0144. Print 2024 Nov 6.

1476-1645

https://pubmed.ncbi.nlm.nih.gov/39226893/?otool=iaurydwlib

https://hdl.handle.net/10137/13551

Burkholderia pseudomallei

Melioidosis

Aminoglycosides

Gentamicins

Phylogeny

Bangladesh

Malaysia

Borneo

Anti-Bacterial Agents

Communicable Diseases

Use of Comparative Genomics To Resolve an Unusual Case of Aminoglycoside Susceptibility in the Melioidosis Pathogen Burkholderia pseudomallei in Bangladesh.

Journal Article

Publication